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Specimen Nr. 02B

Specimen:

Cartilage (Sheep)

Staining:

TEM

Magnification:

73000x

Important structures :

1.Collagen fibrils
2.Elastic fibre
3.Proteoglycans
4.Fibrocyte process
Auf diesem Schnitt sind die kollagenen Fibrillen vorwiegend quer angeschnitten. Dazwischen gelagert sind Anschnitte von elastischen Fasern. Auf dem Querschnitt einer elastischen Faser sind deutlich die 2 Komponenten zu erkennen: die amorphe, mäßig elektronendicht erscheinende Region des Elastinkerns, in deren Peripherie zahlreiche Mikrofilamente angeordnet sind. Die kleinen elektronendichten Partikel mit feinsten fädigen Ausläufern sind das morphologische Äquivalent der Proteoglykane.

Legende:

Collagen fibrils
Elastic fibre
Proteoglycans
Fibrocyte process

Elastic fibre[sl]

1. Amorphous elastin
2. Microfibrils

Transmission electron microscope (TEM)

The sections for examination under the transmission electron microscope are about 0.1µm thick. They are referred to as ultra-thin sections.

To obtain such ultra-thin sections, the tissues are embedded in plastic polymers like epon (instead of paraffin, which is used for examination under the light microscope) after fixation and dehydration. Ultra-thin sections are not stained with dyes, but contrasted with heavy-metal salts. The heavy-metal salts lead to a different electron scatter and thereby create a differentiated blackening of the photographic negative. A common method of creating contrast results with 5% uranyl acetate and lead citrate.

Elastische Fasern setzen sich aus Mikrofibrillen, die aus dem Glykoprotein Fibrillin bestehen, und aus Elastin, welches amorph erscheint, zusammen. Durch die Interaktion von Elastin und Fibrillin kommt es zu den spezifischen Eigenschaften der elastischen Fasern, der Elastizität der Gewebe.
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Magnification:

27600x

Magnification:

73000x

Magnification:

82000x

Magnifications
Collagen fibrils
Collagen fibrils
Elastic fibre
Proteoglycans
Fibrocyte process

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

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