Specimen Nr. 05B

Specimen:

Skin of scrotum (Human being)

Staining:

Orcein

Magnification:

176x

Important structures :

1.Elastic fibres

Die braunrot angefärbten elastischen Fasern des Stratum papillare und Stratum reticulare der Skrotalhaut liegen eingestreut zwischen den kaum angefärbten kollagenen Bindegewebsfasern sowie den glatten Muskelzellen. An der Grenze zur Epidermis sind die elastischen Fasern dünner und oft senkrecht verlaufend, zur Subcutis hin dagegen dicker und meist parallel zur Oberfläche ausgerichtet.

Legende:

Elastic fibres

Loose connective tissue[kr]

1. Fibroblast
2. Fibrocyte
3. Single collagen microfibrils
4. Bundle of collagen microfibrils
5. Elastic fibres

Elastic Fibre Staining

A special staining technique used to identify elastic fibres. The table below shows the dyes which selectively stain elastic fibres.

Dyes

Colours of Elastic Fibres

Orcein

red-brown

Resorcin-fuchsin

blackish-blue to violet

Aldehydfuchsin

red to violet

Combinations with further dyes and staining techniques:

hemalaun

dye for staining cell nuclei
result: Cell nuclei turn intense blue.

nuclear fast red

used as a counterstain with Weigerts elastica dye
result: Cell nuclei turn red.

Die Spezialmethode zur Anfärbung von elastischen Fasern ist die Elastika-Färbung nach Weigert. Farbstoffe sind Orcein oder Resorcinfuchsin - Kernechtrot. Die elastischen Fasern werden dabei braunrot bzw. schwarzblauviolett angefärbt, während retikuläre und kollagene Fasern, Muskelgewebe, Knorpel, sowie das Zytoplasma der Zellen nicht angefärbt werden.

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Magnification:

41x

Magnification:

176x

Magnifications
Elastic fibres
Elastic fibres

HistoNet2000 - Help

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Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
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