Die längs angeschnittene Kollagenfibrille zeigt die aus hellen und dunklen Banden bestehende Querstreifung mit einer Periodizität von 67 nm. Neben der längs angeschnittenen Kollagenfibrille finden sich schräg angeschnittene Kollagenfibrillen und zahlreiche Proteoglykane.

Legende:

	Collagen fibril (sliced longitudinally)
	Collagen fibrils (sliced obliquely)
	Proteoglycans

Transmission electron microscope (TEM)

The sections for examination under the transmission electron microscope are about 0.1µm thick. They are referred to as ultra-thin sections.

To obtain such ultra-thin sections, the tissues are embedded in plastic polymers like epon (instead of paraffin, which is used for examination under the light microscope) after fixation and dehydration. Ultra-thin sections are not stained with dyes, but contrasted with heavy-metal salts. The heavy-metal salts lead to a different electron scatter and thereby create a differentiated blackening of the photographic negative. A common method of creating contrast results with 5% uranyl acetate and lead citrate.

Die Querstreifung der kollagenen Fibrille mit einer Periodizität von 67 nm ergibt sich durch die Einlagerung von Schwermetallsalzen im Zuge der Kontrastierung des Ultradünnschnittes für die elektronenmikroskopische Untersuchung. Die Schwermetallsalze sammeln sich vor allem in Zonen an, in denen die Fibrillenstruktur weniger dicht ist. Solche Zonen entstehen durch die versetzte Anordnung der Tropokollagenmoleküle, die die Grundeinheiten der Mikrofibrillen darstellen.

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

Specimen Nr. 02C

Specimen:

Staining:

Magnification:

Important structures :

Legende:

Transmission electron microscope (TEM)

HistoNet2000 - Help