| 1. | End pieces (epithelial cells) |
| 2. | Extracellular matrix |
| 3. | Excretory duct |
| 4. | Fibrocyte with nucleus |
Dieser Ausschnitt aus dem exokrinen Teil des Pankreas (Bauchspeicheldrüse) zeigt neben den Epithelzellen der Drüsenendstücke vor allem Bindegewebe, das einen Ausführungsgang umgibt. Im lockeren Bindegewebe des Schnittpräparats ist - anders als bei den Fibroblasten der Gewebekultur (siehe Präp. 01A und 01B) - der Zelleib des Fibrozyten klein und spindelförmig. Die extrazelluläre Matrix, von der die Fasern lichtmikroskopisch zu sehen sind, schmiegt sich eng den Fibrozyten an. In ihnen ist der intensiv gefärbte Zellkern (Nucleus) gut zu erkennen.
 | End pieces (epithelial cells) |
 | Extracellular matrix |
 | Excretory duct |
 | Fibrocyte with nucleus |
|
H&E is the most common survey stain, which shows cell nuclei and cytoplasmatic components.
| Structures | Colour |
| Nuclei | blue |
| Cytoplasm | pale red |
| Connective-tissue fibres - Collagen - Reticular - Elastic |
red - pale rose to red |
| Muscle tissue | red |
| Erythrocytes | red-orange |
| Hematoxylin | (e.g. hemalaun, Weigerts or Heidenhains iron-hematoxylin) Hematoxylin is a positively charged dye (at low pH values). It therefore colors negatively charged (basophilic) structures blue, like the DNA of nuclear chromatin, the RNA of rough endoplasmatic reticulum, and the acidic glycosaminoglycans of hyaline cartilage. Hematoxylin used in the preparation of iron varnish is Weigerts or Heidenhains iron-hematoxylin and in the preparation of aluminum varnish is hemalaun. |
| Eosin | Eosin is a negatively charged dye used for counterstaining. It stains all remaining structures different shades of red (acidophilic). |
Thiazinrot |
Thiazinrot behaves similar as eosin. |
Fibroblasten bzw. Fibrozyten synthetisieren die Bausteine der extrazelluläre Matrix der Binde- und Stützgewebe. Diese Bausteine sind Fasern (kollagene , retikuläre und elastische Fasern) und "amorphe" Grundsubstanz. Die lichtmikroskopisch amorph erscheinende Grundsubstanz enthält vor allem Glykosaminoglykane (z.B. Chondroitinsulfat, Keratansulfat und Hyaluronsäure) und Proteoglykane.
1. Organization of the screen surface
Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)
2.Histologic specimen
Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.
3. Complementary information
Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated
4. General Program Functions
Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy
We hope you will enjoy working with HistoNet2000 and learn a lot from it!