Specimen Nr. 05

Specimen:

Head (Rat fetus)

Staining:

Hopa

Magnification:

256x

Important structures :

1.Mesenchymal cell
2.Nucleus
3.Cell process
4.Extracellular matrix

Mesenchymzellen bilden mit ihren langen Zellfortsätzen ein dreidimensionales Netzwerk. In den Lücken befindet sich die von ihnen sezernierte extrazelluläre Matrix, die hauptsächlich aus dem Glykosaminoglykan Hyaluronsäure und Flüssigkeit besteht. Kollagene Faser kommen im mesenchymalen Bindegewebe nur selten vor.

Legende:

Mesenchymal cell
Nucleus
Cell process
Extracellular matrix

Mesenchyme[bu]

1. Mesenchymal cells
2. Intercellular space
3. Capillary

HOPA

Structures Colour
Nuclei brown
Cytoplasm grey-blue
Connective-tissue fibres
- Collagen
- Reticular
- Elastic

blue
blue
-
Myocytes grey-blue to grey-green
Erythrocytes yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Die Mesenchymzellen sind die Zellen des embryonalen Bindegewebes. Sie zeichnen sich durch eine hohe Teilungsrate aus und können sich amöboid zu ihrem Bestimmungsort hin fortbewegen. Als pluripotente Zellen gehen durch Differenzierung aus den Mesenchymzellen verschiedene Gewebe hervor, z.B.:

- Binde- und Stützgewebe
- Glatte und quergestreifte Muskulatur
- Blutzellen
- Blutgefäße
Home
Tutor
Help
Exit
Boxes
Mesenchymal cell
Nucleus
Cell process
Extracellular matrix

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

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