Specimen Nr. 06A

Specimen:

Spleen (Rat)

Staining:

India ink (in vivo), H&E

Magnification:

8x

Important structures :

1.Red pulp of spleen
2.White pulp of spleen
In der Übersicht erkennt man die zwei Hauptbestandteile der Milz: die rote Milzpulpa und die weiße Milzpulpa. Zur weißen Pulpa gehören die dunkler blau gefärbten Milzknötchen, Aggregationen lymphatischer Zellen. Die heller gefärbten und locker strukturierten Areale stellen die rote Milzpulpa dar. Die durch in vivo-Injektion von Tusche zur Phagozytose angeregten Makrophagen sind bei dieser Vergrößerung noch nicht zu erkennen.

Legende:

Red pulp of spleen
White pulp of spleen

Monocytes

Monocytes are part of the immune system – the mononuclear phagocyte system. Chemotactic stimuli cause them to cross the capillary walls and enter the connective tissues, where they differentiate into macrophages. Here they can be found in both mobile and sessile (histiocytes) forms.

Hematoxylin and Eosin (H&E)

H&E is the most common survey stain, which shows cell nuclei and cytoplasmatic components.

Structures Colour
Nuclei blue
Cytoplasm pale red
Connective-tissue fibres
- Collagen
- Reticular
- Elastic

red
-
pale rose to red
Muscle tissue red
Erythrocytes red-orange

Dyes:

Hematoxylin (e.g. hemalaun, Weigerts or Heidenhains iron-hematoxylin)
Hematoxylin is a positively charged dye (at low pH values). It therefore colors negatively charged (basophilic) structures blue, like the DNA of nuclear chromatin, the RNA of rough endoplasmatic reticulum, and the acidic glycosaminoglycans of hyaline cartilage.
Hematoxylin used in the preparation of iron varnish is Weigerts or Heidenhains iron-hematoxylin and in the preparation of aluminum varnish is hemalaun.
Eosin Eosin is a negatively charged dye used for counterstaining. It stains all remaining structures different shades of red (acidophilic).

Thiazinrot

Thiazinrot behaves similar as eosin.

Das Milzparenchym setzt sich aus weißer Milzpulpa (15-20% des gesamten Milzvolumens) und roter Milzpulpa (Hauptbestandteil der Milz) zusammen.

Die weiße Milzpulpa besteht aus lymphatischem Gewebe:

Die rote Milzpulpa besteht aus einem schwammartigen Maschenwerk sternförmiger Retikulumzellen, den Pulpasträngen und aus Sinusoiden.
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Magnification:

8x

Magnification:

32x

Magnification:

128x

Magnifications
Red pulp of spleen
Red pulp of spleen
Red pulp of spleen
White pulp of spleen
White pulp of spleen
White pulp of spleen
White pulp of spleen

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

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