Specimen Nr. 01A

Specimen:

Mesenchyme (Rat fetus)

Staining:

Hopa

Magnification:

3,2x

Important structures :

1.	Tongue germ
2.	Eye germ
3.	Nose germ
4.	Brain germ
5.	Tooth germ (bud stage)
6.	Skin
7.	Mesenchyme
8.	Bone tissue

Das Präparat zeigt einen Frontalschnitt durch den Kopf eines Rattenfetus. Zwischen den Zungen-, Augen-, Nasen-, Gehirn- und Zahnanlagen liegt das embryonale Bindegewebe, das Mesenchym, dessen undifferenzierte extrazelluläre Matrix blaßblau gefärbt ist.

Legende:

	Tongue germ
	Eye germ
	Nose germ
	Brain germ
	Tooth germ (bud stage)
	Skin
	Mesenchyme
	Bone tissue

Mesenchyme[bu]

1.	Mesenchymal cells
2.	Intercellular space
3.	Capillary

HOPA

Structures	Colour
Nuclei	brown
Cytoplasm	grey-blue
Connective-tissue fibres - Collagen - Reticular - Elastic	blue blue -
Myocytes	grey-blue to grey-green
Erythrocytes	yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Mesenchym ist das embryonale Bindegewebe, das vor allem aus dem Mesoderm, dem mittleren Keimblatt entsteht. Am Kopf stammt es teilweise auch aus der Neuralleiste, einem Abkömmling des Ektoderms, des äußeren Keimblatts.
Aus dem Mesenchym entwickeln sich durch Differenzierung alle weiteren Gewebe und Organe mesodermaler Herkunft, wie z.B. Blutgefäße, Blut, Muskulatur, Binde- und Stützgewebe.

Magnification:

3,2x

Magnification:

70x

Magnification:

256x

Tooth germ (bud stage)

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!