Specimen Nr. 02A

Specimen:

Gelatinous connective tissue, Umbilical cord (Human being)

Staining:

Azan

Magnification:

Important structures :

1.	Gelatinous connective tissue
2.	Umbilical artery
3.	Umbilical vein
4.	Amniotic epithel
5.	Slice artifact

Die Nabelschnur, die hier im Querschnitt zu sehen ist, wird von gallertigem Bindegewebe, der sog. Whartonschen Sulze, aufgebaut. Eingebettet in diesem Gallertgewebe liegen die beiden Aa. umbilicales und die V. umbilicalis. Die hellen Spalträume im Gallertgewebe stellen durch das Schneiden des Präparates bedingte Artefakte dar. Die Oberfläche der Nabelschnur wird vom Amnionepithel gebildet.

Legende:

	Gelatinous connective tissue
	Umbilical artery
	Umbilical vein
	Amniotic epithel
	Slice artifact

Gelatinous connective tissue (Umbilical cord)[bu]

1.	Mesenchymal cells
2.	Small collagen fibrils
3.	Gelatinous intercellular substance

Azan (Heidenhain)

Azan dye is a multiple or trichromatic staining method. It differentiates extracellular connective-tissue fibres, tissues, and cell components.

Structures	Colour
Nuclei	red
Cytoplasm	reddish
Connective-tissue fibres - Collagenic - Reticular - Elastic	blue blue unstained or reddish
Muscle tissue	red-orange

Dyes:

Azokarmin	Azokarmin is a red dye used mainly to stain cell nuclei.
Aniline blue- orange G	This dye is used for counterstaining.

Die ca. 50-60 cm lange Nabelschnur führt vom Nabel des Kindes zur fetalen Seite der Plazenta. Sie enthält drei Gefäße: die beiden Aa. umbilicales führen kindliches Blut zur Plazenta, eine V. umbilicalis führt das Blut von der Plazenta, wo ein Austausch von Stoffwechselprodukten und Gasen zwischen mütterlichem und kindlichem Blut stattfindet, zurück zum Kind.

Magnification:

64x

Magnification:

128x

Gelatinous connective tissue

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

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