Specimen Nr. 04A

Specimen:

Loose connective tissue, Lip (Human being)

Staining:

Hemalaun thiacinrot

Magnification:

32x

Important structures :

1.	Lower epithelial layers of lip
2.	Loose fibrous connective tissue
3.	White adipose tissue

Dieser Ausschnitt der Lippe zeigt die unteren Schichten des Oberflächenepithels, die eng mit dem angrenzenden lockeren faserigen Bindegewebe verzahnt sind. Unregelmäßig angeordnete, rosa angefärbte Fasern mit zahlreichen dazwischen gestreuten Zellen, die sich bei dieser Vergrößerung nicht identifizieren lassen, sowie das Vorkommen vieler Blutgefäße, sind für diese Bindegewebsart charakteristisch. Die hellen Areale sind eingestreute Zellen des weißen Fettgewebes.

Legende:

	Lower epithelial layers of lip
	Loose fibrous connective tissue
	White adipose tissue

Loose connective tissue[kr]

1.	Fibroblast
2.	Fibrocyte
3.	Single collagen microfibrils
4.	Bundle of collagen microfibrils
5.	Elastic fibres

Hematoxylin and Eosin (H&E)

H&E is the most common survey stain, which shows cell nuclei and cytoplasmatic components.

Structures	Colour
Nuclei	blue
Cytoplasm	pale red
Connective-tissue fibres - Collagen - Reticular - Elastic	red - pale rose to red
Muscle tissue	red
Erythrocytes	red-orange

Dyes:

Hematoxylin

(e.g. hemalaun, Weigerts or Heidenhains iron-hematoxylin)
Hematoxylin is a positively charged dye (at low pH values). It therefore colors negatively charged (basophilic) structures blue, like the DNA of nuclear chromatin, the RNA of rough endoplasmatic reticulum, and the acidic glycosaminoglycans of hyaline cartilage.
Hematoxylin used in the preparation of iron varnish is Weigerts or Heidenhains iron-hematoxylin and in the preparation of aluminum varnish is hemalaun.

Eosin

Eosin is a negatively charged dye used for counterstaining. It stains all remaining structures different shades of red (acidophilic).

Thiazinrot

Thiazinrot behaves similar as eosin.

Lippen sind außen von verhorntem geschichtetem Plattenepithel, innen von unverhorntem geschichtetem Plattenepithel überzogen. Der Übergang der beiden Epithelarten erfolgt im Lippenrot. Den Mittelteil der Lippe bildet ein vor allem aus lockerem faserigen Bindegewebe und quergestreiftem Muskelgewebe bestehendes Polster.

Magnification:

32x

Magnification:

128x

Lower epithelial layers of lip

Loose fibrous connective tissue

White adipose tissue

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

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