Specimen Nr. 08A

Specimen:

Adipose tissue, Kidney (Rat)

Staining:

Hopa

Magnification:

8x

Important structures :

1.Renal parenchym
2.Ureter
3.Branch of a renal artery
4.Vein
5.Brown adipose tissue
6.White adipose tissue
Dieser Ausschnitt zeigt die Nierenbucht (Sinus renalis) mit dem Nierenbecken (Pelvis renalis). An einer Stelle ist hier Nierenparenchym zu erkennen. Anschnitte von Ästen der Nierenarterie und -venen sowie der Ureterquerschnitt liegen im Fettgewebe des Nierenbeckens. Bereits bei der Übersichtsvergrößerung ist das weiße vom braunen Fettgewebe zu unterscheiden.

Legende:

Renal parenchym
Ureter
Branch of a renal artery
Vein
Brown adipose tissue
White adipose tissue

Adipose tissue[bu]

1. Capillary
2. Nucleus of adipocyte
3. Adipose cell

HOPA

Structures Colour
Nuclei brown
Cytoplasm grey-blue
Connective-tissue fibres
- Collagen
- Reticular
- Elastic

blue
blue
-
Myocytes grey-blue to grey-green
Erythrocytes yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Es gibt zwei Arten von Fettgeweben: Weißes und braunes Fettgewebe. Weißes Fettgewebe ist die Hauptform. Es kann nach der physiologischen Bedeutung als Speicher- oder Baufettgewebe vorliegen. Braunes Fettgewebe spielt vor allem beim Neugeborenen eine Rolle. Im Erwachsenenalter kommt es nur noch an wenigen Stellen (z.B. Nierenbecken, Achselhöhle, Halsregion, Rumpfgegend) in kleinen Mengen vor.
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Magnification:

8x

Magnification:

64x

Magnification:

128x

Magnification:

280x

Magnifications
Renal parenchym
Ureter
Branch of a renal artery
Vein
Brown adipose tissue
Brown adipose tissue
White adipose tissue
White adipose tissue
White adipose tissue

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

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