Specimen Nr. 01A

Specimen:

Hyaline cartilage, Trachea (Human being)

Staining:

H&E

Magnification:

8x

Important structures :

1.Respiratory mucosa
2.Submucosa
3.Hyaline cartilage of the fibromusculocartilagineous layer
4.Perichondrium
5.Shrinkage artifact
6.Tracheal glands
7.Mucous coating
Auf dem senkrechten Schnitt durch die Wand der Trachea sind folgende Schichten zu erkennen: Tunica mucosa respiratoria (Schleimhaut), Tela submucosa (Bindegewebsschicht), Tunica fibromusculocartilaginea (Bindegewebs ? Muskel ? Knorpelschicht) und Tunica adventitia. In der Tela submucosa liegen seromuköse Drüsen, die Glandulae tracheales. Von der Tunica fibromusculocartilaginea sind die hyalinen Knorpelspangen zu sehen.

Legende:

Respiratory mucosa
Submucosa
Hyaline cartilage of the fibromusculocartilagineous layer
Perichondrium
Shrinkage artifact
Tracheal glands
Mucous coating

Hyaline cartilage[bu]

1. Perichondrium
2. Hyaline cartilage
3. Chondrocyte
4. Matrix (interterritorial)

Hematoxylin and Eosin (H&E)

H&E is the most common survey stain, which shows cell nuclei and cytoplasmatic components.

Structures Colour
Nuclei blue
Cytoplasm pale red
Connective-tissue fibres
- Collagen
- Reticular
- Elastic

red
-
pale rose to red
Muscle tissue red
Erythrocytes red-orange

Dyes:

Hematoxylin (e.g. hemalaun, Weigerts or Heidenhains iron-hematoxylin)
Hematoxylin is a positively charged dye (at low pH values). It therefore colors negatively charged (basophilic) structures blue, like the DNA of nuclear chromatin, the RNA of rough endoplasmatic reticulum, and the acidic glycosaminoglycans of hyaline cartilage.
Hematoxylin used in the preparation of iron varnish is Weigerts or Heidenhains iron-hematoxylin and in the preparation of aluminum varnish is hemalaun.
Eosin Eosin is a negatively charged dye used for counterstaining. It stains all remaining structures different shades of red (acidophilic).

Thiazinrot

Thiazinrot behaves similar as eosin.
Der Name Tunica fibromusculocartilaginea bringt zum Ausdruck, daß diese Schicht der Trachealwand aus Bindegewebe ("fibro"), Muskulatur ("musculo") und Knorpel ("cartilaginea") zusammengesetzt ist. Die ventral liegenden hyalinen hufeisenförmigen Knorpelspangen versteifen die Wand, so daß das Lumen trotz wechselnder Drucke bei Ein- und Ausatmung offenbleibt. Dorsal verschließt straffes Bindegewebe und glatte Muskulatur (M. trachealis) den Spalt zwischen den Knorpelspangen. Durch diese Bauweise wird erreicht, daß die Trachea kein starres Rohr ist.
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Magnification:

8x

Magnification:

13x

Magnification:

128x

Magnifications
Respiratory mucosa
Submucosa
Hyaline cartilage of the fibromusculocartilagineous layer
Perichondrium
Shrinkage artifact
Tracheal glands
Mucous coating

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
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