Specimen Nr. 01A

Specimen:

Myenteric neural plexus, Jejunum (Human being)

Staining:

Hopa

Magnification:

11x

Important structures :

1.	Mucosa
2.	Submucosa
3.	Muscular layer
4.	Serosa
5.	Arteriole
6.	Venule
7.	Lymphatic vessel
8.	Myenteric neural plexus

Das Präparat zeigt einen Längsschnitt durch das menschliche Jejunum mit den verschiedenen Schichten der Darmwand. Es sind vegetative Ganglien als Anteile des enterischen Nervensystems zu sehen. Diese finden sich sowohl in der Tela submucosa (Plexus submucosus) als auch in der Tunica muscularis (Plexus myentericus). Letzterer ist leichter zu finden als der Plexus submucosus. In der Tela submucosa sieht man Arteriolen (leeres Lumen, dickere Wand), Venolen (dünnere Wand, Lumen mit Erythrozyten gefüllt) sowie ein Lymphgefäß (leeres Lumen, sehr dünne Wand).

Legende:

	Mucosa
	Submucosa
	Muscular layer
	Serosa
	Arteriole
	Venule
	Lymphatic vessel
	Myenteric neural plexus

Ganglion[bu]

1.	Ganglion cell (neuron)
2.	Satellite cells
3.	Nucleus of Schwann cell
4.	Nerve fibre
5.	Lipofuscin (age pigment)
6.	Capillary
7.	Endoneurium
8.	Axon hillock of a ganglion cell
9.	Slice of ganglion cell (neuron)

HOPA

Structures	Colour
Nuclei	brown
Cytoplasm	grey-blue
Connective-tissue fibres - Collagen - Reticular - Elastic	blue blue -
Myocytes	grey-blue to grey-green
Erythrocytes	yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Das enterische Nervensystem in der Wand des Verdauungstraktes ist neben dem Sympathikus und Parasympathikus der dritte wichtige Teil des vegetativen Nervensystems. Es funktioniert weitgehend autonom, wird aber von Sympathikus und Parasympathikus beeinflußt, und zwar in entgegengesetzter Art und Weise.

Magnification:

11x

Magnification:

28x

Magnification:

44x

Magnification:

102x

Magnification:

176x

Myenteric neural plexus

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!