Specimen Nr. 03B

Specimen:

Sciatic nerve (cross section) (Pig)

Staining:

Azan

Magnification:

280x

Important structures :

1.	Axon
2.	Myelin sheath
3.	Endoneurium
4.	Perineurium
5.	Artificial gap caused by shrinkage
6.	Capillary
7.	Unilocular adipocytes

Die quergeschnittenen Axone werden durch kleine rote Punkte repräsentiert. Sie werden von einem ?leeren? Hof umgeben, der Markscheide, die durch das Einbettungsverfahren herausgelöst ist. Die blauen Ringe, die jeweils Axon plus Markscheide umgeben, sind das Endoneurium.

Legende:

	Axon
	Myelin sheath
	Endoneurium
	Perineurium
	Artificial gap caused by shrinkage
	Capillary
	Unilocular adipocytes

Peripheral nerve (cross section)[bo]

1.	Artery
2.	Vein
3.	Myelinated nerve fibre
4.	Epineurium
5.	Perineurium

Azan (Heidenhain)

Azan dye is a multiple or trichromatic staining method. It differentiates extracellular connective-tissue fibres, tissues, and cell components.

Structures	Colour
Nuclei	red
Cytoplasm	reddish
Connective-tissue fibres - Collagenic - Reticular - Elastic	blue blue unstained or reddish
Muscle tissue	red-orange

Dyes:

Azokarmin	Azokarmin is a red dye used mainly to stain cell nuclei.
Aniline blue- orange G	This dye is used for counterstaining.

Den einzelnen Axonen kann man nicht ansehen, ob es sich um afferente oder um efferente Axone handelt (Überlege, wo in den beiden Fällen jeweils der Zellkörper liegen würde!). Beachte auch, daß die Markscheiden unterschiedlich dick sind und überlege, was für eine physiologische Bedeutung das hat!

Magnification:

40x

Magnification:

280x

Artificial gap caused by shrinkage

Unilocular adipocytes

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!