Das Axon ist ein dünner, rot gefärbter Faden, der nicht durchgehend zu sehen ist. Die Markscheide stellt sich optisch leer dar, weil das Myelin durch das Einbettungsverfahren herausgelöst ist. Die blau gefärbten Strukturen sind die Kollagenfasern des Endoneuriums. Die Schwann'schen Zellen sind nur aufgrund ihrer rot gefärbten Zellkerne zu erkennen (diese sind kaum von Kernen von Fibrozyten zu unterscheiden, an die man auch denken muß).

Legende:

	Axon
	Myelin sheath
	Ranvier node
	Nucleus of Schwann cell

Richardson stain

Semi-thin sections are often stained by Richardson technique with a blue dye, Methylene blue Azure II.

Basophilic and osmiophilic structures stain blue, and metachromatic structures turn red-violet.

Semi-thin sections of tissue specimens are generally prepared for the light microscopic examination before ultra-thin sections are prepared for examination under the transmission electron microscope (TEM). Semi-thin sections are thinner than other sections commonly used for light microscopy.

The embedding agent for TEM examination is a plastic resin, which is harder than paraffin. Since plastic resin cannot be detached easily and without creating artifacts, in contrast to paraffin, there exist only few suitable staining techniques for tissue embedded in plastic resin. Richardson stain is one of them.

Die Axone mit den dicksten Markscheiden sind die Aα -Fasern, die sowohl efferent als auch afferent (proprioceptive Fasern) sein können und im Dienste der Regulation der Motorik stehen (überlege, wo jeweils die neuronalen Zellkörper und wo die Rezeptor-Elemente liegen würden!). Wenig myelinisierte (Aδ-Fasern) oder marklose (C-Fasern) Axone vermitteln afferente nociceptive oder efferente visceromotorische Impulse.

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

Specimen Nr. 04

Specimen:

Staining:

Magnification:

Important structures :

Legende:

Richardson stain

HistoNet2000 - Help