Specimen Nr. 01B

Specimen:

Spinal cord (Cat)

Staining:

Silver

Magnification:

80x

Important structures :

1.	Anterior horn with 3 perikarya of somatomotoric neurons
2.	Anterolateral bundle with 2 myelinated ascending or descending axons

Ausschnitt an der Grenze zwischen grauer Substanz (Vorderhorn) und weißer Substanz. In der weißen Substanz sieht man zahlreiche quergeschnittene Axone (schwarze Punkte) mit dickerer oder dünnerer Myelinscheide (weißer, nicht gefärbter Hof).

Legende:

	Anterior horn with 3 perikarya of somatomotoric neurons
	Anterolateral bundle with 2 myelinated ascending or descending axons

Silver impregnation

In silver impregnation an ammoniacal silver-hydroxide solution is absorbed by tissue structures and precipitated by a reducing agent.

This results in a black stain of:

reticular (“argyrophilic”) fibres
neurofibrils
glial filaments

Den Axonen kann man nicht ansehen, ob sie auf- oder absteigen. Im Vorderseitenstrang kommen als Beispiele für absteigende Axone solche von Pyramidenzellen in der Großhirnrinde und für aufsteigende Axone solche von Neuronen im Hinterhorn der grauen Substanz in Frage (Weiterleitung von nociceptiven Signalen zum Thalamus oder proprioceptiven Signalen zum Kleinhirn).

Magnification:

10x

Magnification:

80x

Magnification:

200x

Anterior horn with 3 perikarya of somatomotoric neurons

Anterolateral bundle with 2 myelinated ascending or descending axons

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!