Specimen Nr. 01C

Specimen:

Spinal cord (Cat)

Staining:

Silver

Magnification:

200x

Important structures :

1.	2 somatomotoric perikarya with cell processes (dendrites and axons cannot be differentiated)
2.	Neuropil
3.	Bundle of motor axons (fila radicularia)
4.	Artificial gap caused by shrinkage
5.	Blood vessel

Ausschnitt an der Grenze zwischen grauer Substanz (Vorderhorn) und weißer Substanz. Es sind ca. 10 Perikaryen von somatomotorischen Neuronen zu sehen, die von artifiziellen Schrumpfspalten umgeben sind. Wegen der Größe der Zellen ist der Zellkern nicht immer getroffen. Deren Axone ziehen als Fila radicularia durch die weiße Substanz aus dem Rückenmark heraus und sind deshalb längs getroffen. Beachte, daß der größte Teil der grauen Substanz von Neuropil eingenommen wird. Dieses besteht aus einem Flechtwerk von Axonen, Dendriten und Gliafortsätzen.

Legende:

	2 somatomotoric perikarya with cell processes (dendrites and axons cannot be differentiated)
	Neuropil
	Bundle of motor axons (fila radicularia)
	Artificial gap caused by shrinkage
	Blood vessel

Silver impregnation

In silver impregnation an ammoniacal silver-hydroxide solution is absorbed by tissue structures and precipitated by a reducing agent.

This results in a black stain of:

reticular (“argyrophilic”) fibres
neurofibrils
glial filaments

Bei den Axonen, die man innerhalb des Neuropils der grauen Substanz sieht, kommen verschiedene Typen in Frage: Es könnte sich im Prinzip handeln um:

Efferente Axone der somatomotorischen Neurone
Axone von Interneuronen (z.B. Renshaw-Zellen)
afferente Axone, die den synaptischen Eingang (Input) der somatomotorischen Neurone oder Interneurone bilden, z. B. Axone von Pyramidenzellen des Motorcortex oder von Spinalganglienzellen)

Magnification:

10x

Magnification:

80x

Magnification:

200x

2 somatomotoric perikarya with cell processes (dendrites and axons cannot be differentiated)

Bundle of motor axons (fila radicularia)

Artificial gap caused by shrinkage

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

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