Information
Drawing
Staining
Knowledge
marked
unmarked

Specimen Nr. 03A

Specimen:

Cerebrum (Human being)

Staining:

Nissl stain

Magnification:

2x

Important structures :

1.Grey matter, cortex
2.White matter, medulla
3.Subarachnoid space deep within a sulcus with blood vessels
Das Präparat zeigt eine von links oben hereinziehende Furche (Sulcus), die oben und unten von je einer Windung (Gyrus) begrenzt wird. Die Rinde (Cortex) ist infolge der dichten Ansammlung von neuronalen Perikarya stärker gefärbt als das Mark. Innerhalb der Rinde erkennt man eine radiäre Gliederung (Columnen), sowie eine horizontale Schichtung. Abgesehen von der zellarmen Schicht I ist es schwierig, die übrigen 5 Schichten im einzelnen abzugrenzen.

Legende:

Grey matter, cortex
White matter, medulla
Subarachnoid space deep within a sulcus with blood vessels

Nissl stain

Dyes: Cresyl violet, Toluidine blue, or Thionin:

These dyes bind to basophilic structures, like RNA and DNA. They especially stain Nissl substance and nucleoli blue or violet.

Home
Tutor
Help
Exit
Boxes

Magnification:

2x

Magnification:

80x

Magnification:

200x

Magnifications
Grey matter, cortex
White matter, medulla
Subarachnoid space deep within a sulcus with blood vessels

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

Cose help