Specimen Nr. 04A

Specimen:

Cerebrum (Human being)

Staining:

Weigert stain of myelin sheath

Magnification:

2x

Important structures :

1.Medulla
2.Cortex, layer I
3.Cortex, layers IV-V (Baillager stripes)
4.Sulcus (subarachnoid space)
Das Präparat zeigt einen von unten hereinziehenden Sulcus, der von 2 Gyri begrenzt wird. Wegen der bevorzugten Anfärbung von Markscheiden erscheint das Mark hier dunkler als die Rinde.

Legende:

Medulla
Cortex, layer I
Cortex, layers IV-V (Baillager stripes)
Sulcus (subarachnoid space)

Myelin-sheath stains

Weigert iron-hematoxylin stain

Structures Colour
Nuclei black to blue-black
Cytoplasm -
Connective-tissue fibres
    - Collagen
    - Reticular
    - Elastic

-
-
black to blue-black
Myocytes black to blue-black
Erythrocytes black to blue-black

Dyes:

Iron-hematoxylin: is a hematoxylin and stains far more intensively than hemalaun. To be able to stain myelin sheaths with Weigert dye, the lipids in the myelin must be preserved while preparing the specimen. The myelin sheaths turn black to blue-black.
Luxol fast blue: Only the neurokeratin remains in normal paraffin fixation of myelin sheaths. Luxol fast blue has a specific affinity to this lipoprotein and stains the myelin sheaths bright blue.
Osmium tetroxide The specimens must be prepared as frozen sections. Osmium tetroxide is deposited on the double bonds of unsaturated fatty acids and produces reaction products which make even the thinnest myelin sheaths visible through black stain.
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Magnification:

2x

Magnification:

25x

Magnification:

65x

Magnifications
Medulla
Cortex, layer I
Cortex, layers IV-V (Baillager stripes)
Cortex, layers IV-V (Baillager stripes)
Sulcus (subarachnoid space)

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

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