Specimen Nr. 04A


Cerebrum (Human being)


Weigert stain of myelin sheath



Important structures :

2.Cortex, layer I
3.Cortex, layers IV-V (Baillager stripes)
4.Sulcus (subarachnoid space)
Das Präparat zeigt einen von unten hereinziehenden Sulcus, der von 2 Gyri begrenzt wird. Wegen der bevorzugten Anfärbung von Markscheiden erscheint das Mark hier dunkler als die Rinde.


Cortex, layer I
Cortex, layers IV-V (Baillager stripes)
Sulcus (subarachnoid space)

Myelin-sheath stains

Weigert iron-hematoxylin stain

Structures Colour
Nuclei black to blue-black
Cytoplasm -
Connective-tissue fibres
    - Collagen
    - Reticular
    - Elastic

black to blue-black
Myocytes black to blue-black
Erythrocytes black to blue-black


Iron-hematoxylin: is a hematoxylin and stains far more intensively than hemalaun. To be able to stain myelin sheaths with Weigert dye, the lipids in the myelin must be preserved while preparing the specimen. The myelin sheaths turn black to blue-black.
Luxol fast blue: Only the neurokeratin remains in normal paraffin fixation of myelin sheaths. Luxol fast blue has a specific affinity to this lipoprotein and stains the myelin sheaths bright blue.
Osmium tetroxide The specimens must be prepared as frozen sections. Osmium tetroxide is deposited on the double bonds of unsaturated fatty acids and produces reaction products which make even the thinnest myelin sheaths visible through black stain.







Cortex, layer I
Cortex, layers IV-V (Baillager stripes)
Cortex, layers IV-V (Baillager stripes)
Sulcus (subarachnoid space)

HistoNet2000 - Help

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Left side: information about the specimen (above) and general program functions (below)

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3. Complementary information

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Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
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