| 1. | Nuclei of neurons (larger) and glial cells (small) |
| 2. | Radially arranged myelinated axons |
| 3. | Branching capillaries with erythrocytes |
 | Nuclei of neurons (larger) and glial cells (small) |
 | Radially arranged myelinated axons |
 | Branching capillaries with erythrocytes |
 |
Weigert iron-hematoxylin stain
| Structures | Colour |
| Nuclei | black to blue-black |
| Cytoplasm | - |
| Connective-tissue fibres - Collagen - Reticular - Elastic |
- - black to blue-black |
| Myocytes | black to blue-black |
| Erythrocytes | black to blue-black |
| Iron-hematoxylin: | is a hematoxylin and stains far more intensively than hemalaun. To be able to stain myelin sheaths with Weigert dye, the lipids in the myelin must be preserved while preparing the specimen. The myelin sheaths turn black to blue-black. |
| Luxol fast blue: | Only the neurokeratin remains in normal paraffin fixation of myelin sheaths. Luxol fast blue has a specific affinity to this lipoprotein and stains the myelin sheaths bright blue. |
| Osmium tetroxide | The specimens must be prepared as frozen sections. Osmium tetroxide is deposited on the double bonds of unsaturated fatty acids and produces reaction products which make even the thinnest myelin sheaths visible through black stain. |
|
Magnification:
2x
Magnification:
25x
Magnification:
65x
1. Organization of the screen surface
Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)
2.Histologic specimen
Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.
3. Complementary information
Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated
4. General Program Functions
Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy
We hope you will enjoy working with HistoNet2000 and learn a lot from it!