Specimen Nr. 07A


Dopaminergic neurons, mesencephalon (Mouse)


Immuncytochemical staining with antibodies against tyrosine hydroxylase



Important structures :

1.Substantia nigra (in tegmentum)
2.Aquaduct of the mesencephalon (midbrain)
Das Präparat zeigt einen Querschnitt durch das Mittelhirn der Maus, das rechts und links von Anteilen des Großhirns begrenzt wird. Speziell angefärbt ist nur die Substantia nigra in der grauen Substanz des Tegmentums rechts und links der Mittellinie.


Substantia nigra (in tegmentum)
Aquaduct of the mesencephalon (midbrain)


Immunohistochemistry is used to confirm the presence of or to identify certain structures or substances in tissue sections which cannot be identified with conventional staining methods. Such structures include: cells, enzymes, hormones, macromolecules like nucleic acids and polysaccharides. The basis of immunohistochemical staining techniques is the antigen-antibody reaction. This method makes it possible to differentiate, for example, various cells in a tissue section according to their different metabolic products or surfaces. Either the metabolic product or a certain surface component serves as the antigen. In the first step, the antigen reacts with a specific antibody. The resulting antigen-antibody complex is invisible. Therefore, in a further step a second antibody bound to an adjuvant is added and binds to the initial antibody (so-called sandwich procedure). The bound adjuvant makes the antigen-antibody complex visible under the microscope and identifies the sought structure. Adjuvants are:

Combination with further dyes or staining techniques:

Das Mittelhirn wird gegliedert in: Tectum (dorsal), Tegmentum und Crura cerebri (basal).






Substantia nigra (in tegmentum)
Aquaduct of the mesencephalon (midbrain)

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

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