1. | Apical cell poles of enterocytes with brush border |
2. | Basal cell poles of enterocytes with vertically arranged cell nuclei |
3. | Goblet cell |
4. | Villous stroma with fibrocytes |
Apical cell poles of enterocytes with brush border | |
Basal cell poles of enterocytes with vertically arranged cell nuclei | |
Goblet cell | |
Villous stroma with fibrocytes |
|
Azan dye is a multiple or trichromatic staining method. It differentiates extracellular connective-tissue fibres, tissues, and cell components.
Structures | Colour |
Nuclei | red |
Cytoplasm | reddish |
Connective-tissue fibres - Collagenic - Reticular - Elastic |
blue blue unstained or reddish |
Muscle tissue | red-orange |
Azokarmin | Azokarmin is a red dye used mainly to stain cell nuclei. |
Aniline blue- orange G |
This dye is used for counterstaining. |
Mikrovilli sind fingerförmige Ausstülpungen der Zellmembran zur Oberflächenvergrößerung. Jeder Mikrovillus besteht aus einem zentralen Bündel aus Aktinfilamenten, die untereinander durch die Proteine Fimbrin und Fascin in ihrer Form gehalten werden. Zur lateralen Oberfläche wird das Aktinbündel durch "Minimyosin" und zum Zytoskelett nach basal durch Spektrin verbunden. Jeder Mikrovillus trägt am apikalen Ende eine amorphe Spitzenregion.
Die Mikrovilli überzieht eine Schicht aus Proteinen, Glykoproteinen und Zuckerresten, die Glykokalix. Sie verleiht den Zellen Antigeneigenschaften. Weiterhin spielt die Glykokalix bei Zellerkennungs- und Adhäsionsmechanismen eine Rolle.
Magnification:
11x
Magnification:
64x
Magnification:
81x
Magnification:
160x
1. Organization of the screen surface
Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)
2.Histologic specimen
Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.
3. Complementary information
Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated
4. General Program Functions
Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy
We hope you will enjoy working with HistoNet2000 and learn a lot from it!