Specimen Nr. 02C

Specimen:

Spleen (Rat)

Staining:

H&E and India-ink stain of macrophages

Magnification:

100x

Important structures :

1.	Capsule
2.	Red pulp with ink-stained macrophages
3.	Central artery
4.	Periarteriolar lymphoid sheath (PALS)
5.	Corona of a secondary lymphoid follicle
6.	Germinal centre of a secondary lymphoid follicle
7.	Marginal zone of white pulp

Die Kapsel der Milz besteht aus straffem, kollagenem Bindegewebe. In der weißen Pulpa sind die periarteriolären lymphatischen Begleitscheiden (PALS), die sich um die Zentralarterien erstrecken, gut zu erkennen. Von diesen zweigen die Lymphfollikel ab. PALS und Follikel werden von der roten Pulpa durch die Marginalzone abgegrenzt.

Legende:

	Capsule
	Red pulp with ink-stained macrophages
	Central artery
	Periarteriolar lymphoid sheath (PALS)
	Corona of a secondary lymphoid follicle
	Germinal centre of a secondary lymphoid follicle
	Marginal zone of white pulp

Hematoxylin and Eosin (H&E)

H&E is the most common survey stain, which shows cell nuclei and cytoplasmatic components.

Structures	Colour
Nuclei	blue
Cytoplasm	pale red
Connective-tissue fibres - Collagen - Reticular - Elastic	red - pale rose to red
Muscle tissue	red
Erythrocytes	red-orange

Dyes:

Hematoxylin

(e.g. hemalaun, Weigerts or Heidenhains iron-hematoxylin)
Hematoxylin is a positively charged dye (at low pH values). It therefore colors negatively charged (basophilic) structures blue, like the DNA of nuclear chromatin, the RNA of rough endoplasmatic reticulum, and the acidic glycosaminoglycans of hyaline cartilage.
Hematoxylin used in the preparation of iron varnish is Weigerts or Heidenhains iron-hematoxylin and in the preparation of aluminum varnish is hemalaun.

Eosin

Eosin is a negatively charged dye used for counterstaining. It stains all remaining structures different shades of red (acidophilic).

Thiazinrot

Thiazinrot behaves similar as eosin.

Capsule

Red pulp with ink-stained macrophages

Central artery

Periarteriolar lymphoid sheath (PALS)

Corona of a secondary lymphoid follicle

Germinal centre of a secondary lymphoid follicle

Marginal zone of white pulp

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

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