Direkt unter der Kapsel befindet sich der lymphozytenarme Randsinus, in den sich die Lymphe aus den afferenten Lymphgefäßen ergießt. Im Randsinus treten Retikulumzellen und Retikulinfasern deutlich hervor. Sie bilden im Spaltraum des Randsinus ein lockeres Netzwerk. Unter dem Randsinus befindet sich die Rinde (Cortex), in der sich dicht gepackt zahlreiche Lymphozyten mit dazwischengestreuten Retikulumzellen befinden.

Legende:

	Adipose tissue
	Capsule
	Marginal sinus
	Cortex
	Nucleus of a lymphocyte
	Nucleus of a reticulum cell
	Reticular fibers

Hematoxylin and Eosin (H&E)

H&E is the most common survey stain, which shows cell nuclei and cytoplasmatic components.

Structures	Colour
Nuclei	blue
Cytoplasm	pale red
Connective-tissue fibres - Collagen - Reticular - Elastic	red - pale rose to red
Muscle tissue	red
Erythrocytes	red-orange

Dyes:

Hematoxylin

(e.g. hemalaun, Weigerts or Heidenhains iron-hematoxylin)
Hematoxylin is a positively charged dye (at low pH values). It therefore colors negatively charged (basophilic) structures blue, like the DNA of nuclear chromatin, the RNA of rough endoplasmatic reticulum, and the acidic glycosaminoglycans of hyaline cartilage.
Hematoxylin used in the preparation of iron varnish is Weigerts or Heidenhains iron-hematoxylin and in the preparation of aluminum varnish is hemalaun.

Eosin

Eosin is a negatively charged dye used for counterstaining. It stains all remaining structures different shades of red (acidophilic).

Thiazinrot

Thiazinrot behaves similar as eosin.

Mehrere afferente Lymphgefäße, die in verschiedenen Geweben ihren Ursprung haben können, sammeln sich in einem Lymphknoten und konzentrieren so Antigene (z.B. Bakterien, Tumorzellen) aus einem großen Bereich auf kleinem Raum. Da sich in der afferenten Lymphe nur wenige Zellen befinden, gilt folgende Charakterisierung: die afferente Lymphe ist reich an Antigen und arm an Zellen. Tumorzellen können über die afferente Lymphe in den Lymphknoten gelangen. Dort heften sie sich an den Sinuswänden fest, proliferieren, wodurch sich Metastasen bilden können. Deshalb ist die Kenntnis der Lage von regionären Lymphknoten für die Diagnostik und die Therapie von Tumoren wichtig.

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

Specimen Nr. 01E

Specimen:

Staining:

Magnification:

Important structures :

Legende:

Hematoxylin and Eosin (H&E)

Dyes:

HistoNet2000 - Help