Specimen Nr. 01D

Specimen:

Lymph node (Dog)

Staining:

H&E

Magnification:

100x

Important structures :

1.Medullary cord of lymph node
2.Medullary sinus of lymph node
3.Arteriole
Von den Intermediärsinus des Cortex (Rinde) gelangt die afferente Lymphe in die Marksinus. Die Marksinus liegen zwischen den zellreichen Marksträngen. Sie werden vom dreidimensionalen Maschenwerk der Retikulumzellen durchzogen, so daß eine Art Reusensystem entsteht. Durch die Marksinus sickert die Lymphe auf ihrem Weg vom Cortex (Rinde) und den Intermediärsinus zu den efferenten Lymphgefäßen am Hilum. Weiterhin sind im Mark große Blutgefäße zu beobachten.

Legende:

Medullary cord of lymph node
Medullary sinus of lymph node
Arteriole

Vessels of lymph node[wh]

1. Capsule
2. Trabecula
3. Marginal sinus
4. Cortex
5. Medullary cord of lymph node
6. Artery
7. Vein

Hematoxylin and Eosin (H&E)

H&E is the most common survey stain, which shows cell nuclei and cytoplasmatic components.

Structures Colour
Nuclei blue
Cytoplasm pale red
Connective-tissue fibres
- Collagen
- Reticular
- Elastic

red
-
pale rose to red
Muscle tissue red
Erythrocytes red-orange

Dyes:

Hematoxylin (e.g. hemalaun, Weigerts or Heidenhains iron-hematoxylin)
Hematoxylin is a positively charged dye (at low pH values). It therefore colors negatively charged (basophilic) structures blue, like the DNA of nuclear chromatin, the RNA of rough endoplasmatic reticulum, and the acidic glycosaminoglycans of hyaline cartilage.
Hematoxylin used in the preparation of iron varnish is Weigerts or Heidenhains iron-hematoxylin and in the preparation of aluminum varnish is hemalaun.
Eosin Eosin is a negatively charged dye used for counterstaining. It stains all remaining structures different shades of red (acidophilic).

Thiazinrot

Thiazinrot behaves similar as eosin.
Da in der efferenten Lymphe in der Hauptsache Lymphozyten den Lymphknoten verlassen, gilt folgende Charakterisierung: die efferente Lymphe ist arm an Antigen und reich an Zellen.

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Magnification:

10x

Magnification:

25x

Magnification:

100x

Magnification:

100x

Magnification:

400x

Magnifications
Medullary cord of lymph node
Medullary cord of lymph node
Medullary cord of lymph node
Medullary sinus of lymph node
Medullary sinus of lymph node
Medullary sinus of lymph node
Medullary sinus of lymph node
Arteriole
Arteriole
Arteriole

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

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