|3.||Lumen of a high endothelial venule (HEV)|
|4.||Lymphocyte in lumen of a HEV|
|5.||Lymphocyte on endothelium of HEV|
|6.||Lymphocyte in endothelium of a HEV|
|7.||Lymphocyte in paracortical region|
|Lumen of a high endothelial venule (HEV)|
|Lymphocyte in lumen of a HEV|
|Lymphocyte on endothelium of HEV|
|Lymphocyte in endothelium of a HEV|
|Lymphocyte in paracortical region|
For semi-thin sections, the tissue is not embedded in paraffin (as is otherwise common for light microscopy), but in acrylic or epoxy resins, which are harder embedding substances, and permit thinner slices, so-called semi-thin slices of about 0.5 – max. 2µm in thickness, to be produced. After embedding in paraffin, a section is > 3µm thick. Semi-thin sections have better resolution under a light microscope than the thicker paraffin sections.
Embedding in epoxy resins is always performed when tissues are to be examined under a transmission electron microscope (TEM). Semi-thin sections are used to study the specimen under the light microscope before being examined under the transmission electron microscope (TEM).
Toluidine blue is primarily used to stain semi-thin sections since most dyes do not sufficiently infiltrate the epoxy resin. Toluidine blue stains various biologic components to different shades of blue.
1. Organization of the screen surface
Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)
Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.
3. Complementary information
Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated
4. General Program Functions
Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy
We hope you will enjoy working with HistoNet2000 and learn a lot from it!