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Specimen Nr. 04B

Specimen:

Lingual tonsil (Human being)

Staining:

Hopa

Magnification:

25x

Important structures :

1.Nonkeratinized stratified squamous epithelium of tongue
2.Germinal centre of a secondary lymphoid follicle
3.Corona of a secondary lymphoid follicle
4.Interfollicular area
5.Seromucous glands
6.Skeletal striated muscle of tongue
Die Zungenmandel (Tonsilla lingualis) besitzt Lymphfollikel, die bevorzugt aus B-Lymphozyten bestehen und in der Regel Sekundärfollikel sind, also ein Keimzentrum und eine Korona enthalten. Zwischen den Follikeln befindet sich die Interfollikularregion, in der sich T-Lymphozyten und hochendotheliale Venulen (HEV) befinden. Hier ist die Eintrittspforte von Lymphozyten, die aus der Blutbahn in die Tonsillen einwandern.

Legende:

Nonkeratinized stratified squamous epithelium of tongue
Germinal centre of a secondary lymphoid follicle
Corona of a secondary lymphoid follicle
Interfollicular area
Seromucous glands
Skeletal striated muscle of tongue

Lingual tonsil[bu]

1. Stratified squamous epithelium
2. Epithelial invagination
3. Lymphocytic diapedesis
4. Lymphoid nodule
5. Mucous salivary gland
6. Excretory duct

HOPA

Structures Colour
Nuclei brown
Cytoplasm grey-blue
Connective-tissue fibres
- Collagen
- Reticular
- Elastic
blue
blue
-
Myocytes grey-blue to grey-green
Erythrocytes yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Die Zungenmandel ermöglicht die Antigenaufnahme aus Luft und Nahrung und die Auslösung von Immunreaktionen am Beginn des Atem- und Verdauungstrakts. Durch den ständigen Einstrom von Lymphozyten durch die hochendothelialen Venulen (HEV) in der Interfollikularregion und durch die aus der Tonsille auf dem Lymphweg auswandernden Lymphozyten ist die Zungenmandel in das gesamte Immunsystem integriert.
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Magnification:

10x

Magnification:

25x

Magnification:

100x

Magnifications
Nonkeratinized stratified squamous epithelium of tongue
Nonkeratinized stratified squamous epithelium of tongue
Germinal centre of a secondary lymphoid follicle
Corona of a secondary lymphoid follicle
Interfollicular area
Seromucous glands
Skeletal striated muscle of tongue

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

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Help: Instructions for Use appear
Exit: closes down the HistoNet program
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VM: provides virtual microscopy

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