Specimen Nr. 03A

Specimen:

Peyer's patches, small intestine (Rat), subpopulation of B lymphocytes

Staining:

Immunehistochemical peroxidase technique, nucleus stained with hemalaun

Magnification:

50x

Important structures :

1.Intestinal lumen
2.Secondary follicle
3.Area of M (microfold) cells
4.Interfollicular area
5.B lymphocytes in the lamina propria
Mit einem Antikörper sind immunhistochemisch in braun B-Lymphozyten dargestellt. Sie befinden sich vor allem in den Sekundärfollikeln. Es werden aber auch einige im Dom-Areal und in der Interfollikularregion beobachtet. Außerdem finden sich verstreut liegende B-Lymphozyten in der Lamina propria der Krypten und Zotten.

Legende:

Intestinal lumen
Secondary follicle
Area of M (microfold) cells
Interfollicular area
B lymphocytes in the lamina propria

Localisation of organs of immune-lymphatic system[we]

1. Pharyngeal tonsil
2. Palatine tonsil
3. Lymph node
4. Lymphatic vessels
5. Spleen
6. Peyer's patches
7. Thymus

Immunohistochemestry

Immunohistochemistry is used to confirm the presence of or to identify certain structures or substances in tissue sections which cannot be identified with conventional staining methods. Such structures include: cells, enzymes, hormones, macromolecules like nucleic acids and polysaccharides. The basis of immunohistochemical staining techniques is the antigen-antibody reaction. This method makes it possible to differentiate, for example, various cells in a tissue section according to their different metabolic products or surfaces. Either the metabolic product or a certain surface component serves as the antigen. In the first step, the antigen reacts with a specific antibody. The resulting antigen-antibody complex is invisible. Therefore, in a further step a second antibody bound to an adjuvant is added and binds to the initial antibody (so-called sandwich procedure). The bound adjuvant makes the antigen-antibody complex visible under the microscope and identifies the sought structure. Adjuvants are:

Combination with further dyes or staining techniques:

In den Sekundärfollikeln der Peyerschen Platten werden ständig Zellen gebildet, die sich zu Plasmazellen und Gedächtnis-B-Lymphozyten entwickeln. Außerdem wandern kontinuierlich aus dem Blut B- und T-Lymphozyten über die hochendothelialen Venulen (HEV) in die Peyerschen Platten ein.
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Magnification:

50x

Magnification:

500x

Magnifications
Intestinal lumen
Secondary follicle
Secondary follicle
Area of M (microfold) cells
Area of M (microfold) cells
Interfollicular area
B lymphocytes in the lamina propria
B lymphocytes in the lamina propria
B lymphocytes in the lamina propria

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

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