Specimen Nr. 02C

Specimen:

Femoral artery and vein (Rabbit)

Staining:

Aldehyde fuchsin

Magnification:

81x

Important structures :

1.Lumen of artery
2.Lumen of vein
3.Membrana elastica interna of artery
4.Tunica media of artery
5.Membrana elastica externa of artery
6.Tunica adventitia of artery
7.Tunica intima of vein
8.Tunica media and tunica adventitia of vein
9.Connective tissue between artery and vein

Legende:

Lumen of artery
Lumen of vein
Membrana elastica interna of artery
Tunica media of artery
Membrana elastica externa of artery
Tunica adventitia of artery
Tunica intima of vein
Tunica media and tunica adventitia of vein
Connective tissue between artery and vein

wall of an artery (muscular type)[wh]

1. Intima
2. Tunica media
3. Adventitia
4. Endothelium
5. Membrana elastica interna

Elastic Fibre Staining

A special staining technique used to identify elastic fibres. The table below shows the dyes which selectively stain elastic fibres.

Dyes

Colours of Elastic Fibres

Orcein

red-brown

Resorcin-fuchsin

blackish-blue to violet

Aldehydfuchsin

red to violet

Combinations with further dyes and staining techniques:

hemalaun

dye for staining cell nuclei
result: Cell nuclei turn intense blue.

nuclear fast red

used as a counterstain with Weigerts elastica dye
result: Cell nuclei turn red.

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Magnification:

13x

Magnification:

50x

Magnification:

81x

Magnification:

202x

Magnifications
Lumen of artery
Lumen of vein
Membrana elastica interna of artery
Tunica media of artery
Membrana elastica externa of artery
Tunica adventitia of artery
Tunica intima of vein
Tunica media and tunica adventitia of vein
Connective tissue between artery and vein

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

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