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Specimen Nr. 04A

Specimen:

Stomach, pylorus (Human being)

Staining:

PAS-hemalaun

Magnification:

4x

Important structures :

1.Mucosa
2.Submucosa
3.Muscular layer
4.Serosa

Legende:

Mucosa
Submucosa
Muscular layer
Serosa

Mucosa of stomach[bo]

1. Mucous membrane
2. Gastric areas (areas of mucous membrane)
3. Gastric pits
4. Gastric glands
5. Lamina muscularis of mucosa

PAS-Reaction

Perjodic Acid Schiff Reaction:

Structures containing carbohydrates, like glycogen and reticular fibres containing lots of glycoprotein, basal membranes, and mucins stain pink to violet. Perjodic acid splits the carbon bonds between two neighboring secondary alcohols. Then the alcohol groups are oxidized to aldehydes and are stained reddish by Schiff reagent.

Combinations with further dyes or staining techniques:

Hemalaun / Toluidine blue / Aurantia These dyes are used to stain cell nuclei.
Result: Cell nuclei turn bright blue to dark blue, depending on the dye used.
Luxol fast blue This dye is used to stain myelin sheaths. The dye has a special affinity to myelin.
Result: Myelin sheaths turn bright blue, grey matter turns pale green, and cell nuclei turn dark blue
Home
Tutor
Help
Exit
Boxes

Magnification:

4x

Magnification:

16x

Magnifications
Mucosa
Submucosa
Muscular layer
Serosa

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

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