Specimen Nr. 04A

Specimen:

Ileum (Human being)

Staining:

van Gieson

Magnification:

4x

Important structures :

1.Plica circularis (Kerckring's valve)
2.Mucosa
3.Villi and crypts of mucous membrane
4.Submucosa
5.Muscular layer
6.Circular layer of the muscular layer
7.Longitudinal layer of muscular layer
8.Myenteric neural plexus (in connective tissue between two muscular layers )
9.Serosa
10.Blood vessel

Legende:

Plica circularis (Kerckring's valve)
Mucosa
Villi and crypts of mucous membrane
Submucosa
Muscular layer
Circular layer of the muscular layer
Longitudinal layer of muscular layer
Myenteric neural plexus (in connective tissue between two muscular layers )
Serosa
Blood vessel

Ileum (portion of wall)[bo]

1. Plica circularis (Kerckring's valve)
2. Lamina propria of mucosa
3. Lamina muscularis of mucosa
4. Submucosa
5. Muscular layer
  1. circular layer
  2. longitudinal layer
6. Subserosal layer
7. Serosa
8. Villi
9. Intestinal crypts (glands of Lieberkühn)
10. Lymphoid follicle

Giesons trichromatic stain

This is the dye most commonly used to stain connective tissue.

Structures Colour
Nuclei black-brown
Cytoplasm yellow-brown
Connective-tissue fibres
- Collagen
- Reticular
- Elastic

red
-
yellow
Muscle tissue yellow
Erythrocytes yellow

Dyes:

Weigerts iron hematoxylin This dye is one of the hematoxylins, but stains much more intensively than hemalaun. It is used with van Gieson’s dye to stain cell nuclei.
Picro-fuchsin or Picro-thiacid mixture This dye solution simultaneously stains connective tissue red and muscle tissue yellow (simultaneous stain).
Home
Tutor
Help
Exit
Boxes

Magnification:

4x

Magnification:

28x

Magnification:

64x

Magnification:

160x

Magnification:

64x

Magnifications
Plica circularis (Kerckring's valve)
Mucosa
Villi and crypts of mucous membrane
Villi and crypts of mucous membrane
Submucosa
Muscular layer
Circular layer of the muscular layer
Longitudinal layer of muscular layer
Myenteric neural plexus (in connective tissue between two muscular layers )
Serosa
Blood vessel

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

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