Specimen Nr. 01A

Specimen:

Liver (Pig)

Staining:

Hopa

Magnification:

26x

Important structures :

1.Central vein
2.Periportal space
3.Intralobular sinusoids
4.Hepatocytes
5.Interlobular conective tissue

Legende:

Central vein
Periportal space
Intralobular sinusoids
Hepatocytes
Interlobular conective tissue

Lobule of liver (section)[bo]

1. Interlobular artery
2. Interlobular vein
3. Interlobular bile duct
4. Sinusoid
5. Central vein
6. Hepatocytes
7. Kupffer cells

HOPA

Structures Colour
Nuclei brown
Cytoplasm grey-blue
Connective-tissue fibres
- Collagen
- Reticular
- Elastic

blue
blue
-
Myocytes grey-blue to grey-green
Erythrocytes yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Home
Tutor
Help
Exit
Boxes

Magnification:

26x

Magnification:

70x

Magnification:

160x

Magnification:

70x

Magnification:

202x

Magnifications
Central vein
Periportal space
Periportal space
Intralobular sinusoids
Intralobular sinusoids
Hepatocytes
Hepatocytes
Hepatocytes
Interlobular conective tissue
Interlobular conective tissue
Interlobular conective tissue

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

Cose help