Specimen Nr. 01C

Specimen:

Tongue (Rabbit)

Staining:

Hopa

Magnification:

81x

Important structures :

1.Taste bud
2.Epithelial furrows of a foliate papilla
3.Furrow between two foliate papillae
4.Blood capillaries (lumina)
5.Connective tissue papilla
Die stärkere Vergrößerung zeigt die basalen Abschnitte zweier benachbarter Papillae foliatae, die durch einen Graben voneinander getrennt sind. Mehrere nebeneinander liegende Geschmacksknospen, die die volle Epithelhöhe einnehmen, sind zu sehen. Die tief ins Epithel eindringenden Bindegewebspapillen enthalten zahlreiche Blutkapillaren.

Legende:

Taste bud
Epithelial furrows of a foliate papilla
Furrow between two foliate papillae
Blood capillaries (lumina)
Connective tissue papilla

Taste bud[ju]

1. Supporting cell
2. Intermediate cell
3. Chemoreceptor cell
4. Superficial epithelial cell
5. Basal cell
6. Taste pore
7. Microvilli
8. Synapse
9. Afferent fibre

HOPA

Structures Colour
Nuclei brown
Cytoplasm grey-blue
Connective-tissue fibres
- Collagen
- Reticular
- Elastic

blue
blue
-
Myocytes grey-blue to grey-green
Erythrocytes yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Geschmacksknospen (Caliculi gustatorii) sind Chemorezeptoren, die sich in den vorderen zwei Drittel der Zunge im geschichteten Plattenepithel befinden. Beim Menschen kommen sie vor allem in den Papillae vallatae und den Papillae fungiformes vor. Ihr Vorkommen in den Papillae foliatae ist typisch für das Kaninchen, von dem dieses Präparat stammt. Die Chemorezeptoren können vier Hauptkategorien von Geschmacksempfindungen wahrnehmen: sauer, süß, bitter und salzig.
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Magnification:

13x

Magnification:

32x

Magnification:

81x

Magnification:

202x

Magnifications
Taste bud
Epithelial furrows of a foliate papilla
Furrow between two foliate papillae
Blood capillaries (lumina)
Blood capillaries (lumina)
Blood capillaries (lumina)
Connective tissue papilla

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

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Help: Instructions for Use appear
Exit: closes down the HistoNet program
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VM: provides virtual microscopy

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