Die stärkere Vergrößerung zeigt mehrere helle Geschmacksknospen (Caliculi gustatorii), die in das Oberflächenepithel zweier benachbarter Papillae foliatae eingelagert sind. Bei einer dieser Geschmacksknospen ist der Geschmacksporus angeschnitten, in welchen die sich stark verjüngenden Enden, die sog. Geschmacksstiftchen, der Geschmackszellen hineinragen. Die Geschmackszellen besitzen längliche schmale Kerne. Daneben sind zahlreiche große, helle Kerne zu beobachten, die in verschiedener Höhe liegen und keine Sinneszellen sind. Von den basal liegenden Zellen mit rundlichem Zellkern der Geschmacksknospe sollen die übrigen Zellarten abstammen. Diese Zellen werden auch Basalzellen genannt.

Legende:

	Furrow between two neighbouring foliate papillae
	Slice of stratified papillary epithelium
	Connective tissue
	Taste pore
	Supporting cell
	Gustatory epithelial cell
	Shrinkage artifact

HOPA

Structures	Colour
Nuclei	brown
Cytoplasm	grey-blue
Connective-tissue fibres - Collagen - Reticular - Elastic	blue blue -
Myocytes	grey-blue to grey-green
Erythrocytes	yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Die Geschmacksknospen setzen sich aus verschiedenen zwiebelschalenartig angeordneten Zellen zusammen. Für die Geschmacksempfindung sind die Geschmackszellen, Chemorezeptoren, verantwortlich. Ihre Geschmacksstiftchen sind elektronenmikroskopisch mit Filamenten gefüllte apikale Zellfortsätze, deren Enden sich zu einem dichten Mikrovillisaum aufsplittern („Geschmackspinsel“).

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

Specimen Nr. 01D

Specimen:

Staining:

Magnification:

Important structures :

Legende:

HOPA

Dyes:

HistoNet2000 - Help