Specimen Nr. 06A

Specimen:

Skin of axilla (Human being)

Staining:

van Gieson

Magnification:

6x

Important structures :

1.Epidermis
2.Corium (dermis)
3.Subcutaneous tissue
4.Hair follicle (cross section)
5.Apocrine sweat glands (axillary sweat glands)
6.Eccrine sweat glands

Legende:

Epidermis
Corium (dermis)
Subcutaneous tissue
Hair follicle (cross section)
Apocrine sweat glands (axillary sweat glands)
Eccrine sweat glands

Apocrine secretion, sweat gland (Skin of axilla)[bu]

1. Constricting drop of secretion
2. Basement membrane
3. Myoepithelial cell
4. Glandular cell with double nucleus
5. Nucleus of myoepithelial cell
6. Capillary

Giesons trichromatic stain

This is the dye most commonly used to stain connective tissue.

Structures Colour
Nuclei black-brown
Cytoplasm yellow-brown
Connective-tissue fibres
- Collagen
- Reticular
- Elastic

red
-
yellow
Muscle tissue yellow
Erythrocytes yellow

Dyes:

Weigerts iron hematoxylin This dye is one of the hematoxylins, but stains much more intensively than hemalaun. It is used with van Gieson’s dye to stain cell nuclei.
Picro-fuchsin or Picro-thiacid mixture This dye solution simultaneously stains connective tissue red and muscle tissue yellow (simultaneous stain).

Home
Tutor
Help
Exit
Boxes

Magnification:

6x

Magnification:

13x

Magnification:

32x

Magnification:

41x

Magnification:

70x

Magnifications
Epidermis
Epidermis
Corium (dermis)
Subcutaneous tissue
Hair follicle (cross section)
Hair follicle (cross section)
Apocrine sweat glands (axillary sweat glands)
Eccrine sweat glands

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

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