Specimen Nr. 01A

Specimen:

Rat fetus

Staining:

Hopa

Magnification:

2x

Important structures :

1.Tongue germ
2.Eye germ
3.Nose germ
4.Brain germ
5.Mandibula germ
6.Maxilla germ
7.Tooth germ (bud stage)
8.Dental lamella
9.Meckel cartilage
10.Mesenchyme
Das Präparat zeigt einen Frontalschnitt durch den Kopf eines Rattenfetus. Zwischen den Zungen-, Augen-, Nasen-, Gehirn- und Zahnanlagen liegt das embryonale Bindegewebe, das Mesenchym, dessen undifferenzierte extrazelluläre Matrix blaßblau gefärbt ist.

Legende:

Tongue germ
Eye germ
Nose germ
Brain germ
Mandibula germ
Maxilla germ
Tooth germ (bud stage)
Dental lamella
Meckel cartilage
Mesenchyme

HOPA

Structures Colour
Nuclei brown
Cytoplasm grey-blue
Connective-tissue fibres
- Collagen
- Reticular
- Elastic

blue
blue
-
Myocytes grey-blue to grey-green
Erythrocytes yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Knochen kann auf zwei Wegen gebildet werden:
  1. Desmale (direkte) Ossifikation:
    Die Matrix, die von den Osteoblasten sezerniert wird, wird direkt mineralisiert. Eine Reihe von flachen Schädelknochen und die Clavicula entstehen durch direkte Ossifikation.
  2. Chondrale (indirekte) Ossifikation:
    Das Skelettelement ist zunächst knorpelig angelegt. Durch komplexe Auf- und Abbauvorgänge wird es zu Knochen umgebaut. Durch chondrale Ossifikation entstehen die meisten Knochen des Körpers, wie z.B. die Extremitätenknochen oder die Wirbel.
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Magnification:

2x

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250x

Magnifications
Tongue germ
Eye germ
Nose germ
Brain germ
Mandibula germ
Maxilla germ
Tooth germ (bud stage)
Dental lamella
Meckel cartilage
Mesenchyme

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

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