Specimen Nr. 08

Specimen:

Exocrine cell of pancreas (Bat)

Staining:

TEM

Magnification:

11000x

Important structures :

1.Nucleus of exocrine pancreatic cell (end piece)
2.Nucleolus
3.Rough endoplasmatic reticulum
4.Golgi apparatus
5.Zymogen granules (apical cell pole)
6.Mitochondrion
7.Lumen of serous end piece
8.Nucleus of centroacinar cell
Die exokrinen Drüsenzellen der serösen Endstücke der Bauchspeicheldrüse lassen eine polare Anordnung der Zellorganellen erkennen, die an der Bildung und Abgabe des Sekrets beteiligt sind. Im basalen Teil der Zelle liegen die parallel angeordneten Zisternen des rauhen endoplasmatischen Retikulums in Stapeln übereinander. Im Golgi - Komplex wird die im rauhen endoplasmatischen Retikulum begonnene Synthese weitergeführt. Die Syntheseprodukte werden über Zymogengranula in das Drüsenlumen abgegeben. Diese liegen apikal. Mitochondrien liefern die Energie für diese Stoffwechselvorgänge.

Legende:

Nucleus of exocrine pancreatic cell (end piece)
Nucleolus
Rough endoplasmatic reticulum
Golgi apparatus
Zymogen granules (apical cell pole)
Mitochondrion
Lumen of serous end piece
Nucleus of centroacinar cell

End piece of pancreas with intercalated duct[bo]

1. Secretory acinar cell
2. Zymogen granules
3. Centroacinar cells
4. Basement membrane
5. Intercalated duct

Transmission electron microscope (TEM)

The sections for examination under the transmission electron microscope are about 0.1µm thick. They are referred to as ultra-thin sections.

To obtain such ultra-thin sections, the tissues are embedded in plastic polymers like epon (instead of paraffin, which is used for examination under the light microscope) after fixation and dehydration. Ultra-thin sections are not stained with dyes, but contrasted with heavy-metal salts. The heavy-metal salts lead to a different electron scatter and thereby create a differentiated blackening of the photographic negative. A common method of creating contrast results with 5% uranyl acetate and lead citrate.

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Nucleus of exocrine pancreatic cell (end piece)
Nucleolus
Rough endoplasmatic reticulum
Rough endoplasmatic reticulum
Golgi apparatus
Zymogen granules (apical cell pole)
Zymogen granules (apical cell pole)
Mitochondrion
Mitochondrion
Lumen of serous end piece
Nucleus of centroacinar cell

HistoNet2000 - Help

1. Organization of the screen surface

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Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

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3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
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