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Specimen Nr. 05

Specimen:

Glomerulus (Rat)

Staining:

SEM

Magnification:

5000x

Important structures :

1.Cell body of a podocyte
2.Process of podocyte
3.Capillary loops
Die rasterelektronenmikroskopische Aufnahme zeigt zwei benachbarte Kapillarschlingen eines Rattenglomerulus. Rechts liegt der Zellkörper eines Podozyten, dessen primäre und sekundäre Fortsätze ein komplexes Verzweigungsmuster auf der Oberfläche der Kapillarschlingen bilden. Dabei lassen die Zwischenräume eine sehr große Fläche von Filtrationsspalten offen, durch die das Glomerulusfiltrat in den Bowman - Kapselraum gelangt.

Legende:

Cell body of a podocyte
Process of podocyte
Capillary loops

Renal corpuscle and juxtaglomerular complex

1. Afferent arteriole
2. Efferent arteriole
3. Distal tubule
4. Macula densa
5. Mesangial cells
6. Bowman capsular space
7. Glomerular capillaries
8. Podocytes
9. Urinary pole

Scanning electron microscope (SEM)

It is not necessary to prepare ultra-thin sections for examinations under the scanning electron microscope because the electron beam does not penetrate the object, in contrast to transmission electron microscope (TEM).

The SEM image is created directly by a point-to-point visualization of the surface details of the specimen. To achieve this effect, a very thin electron beam scans the surface of the specimen line by line. Each surface point emits secondary electrons whose different intensities are measured by a detector.

When preparing tissue specimens for a scanning electron microscopic examination, the specimens are dried instead of embedded and sliced after fixation and dehydration.

The dehydration technique commonly used today is critical-point drying. The dried specimens are then coated with gold in a vacuum, the so-called sputtering apparatus. This covers the specimen with a surface which can emit secondary electrons.

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Cell body of a podocyte
Process of podocyte
Process of podocyte
Process of podocyte
Process of podocyte
Capillary loops
Capillary loops
Capillary loops

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

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