Specimen Nr. 02D

Specimen:

Pituitary gland (Human being)

Staining:

Azan

Magnification:

80x

Important structures :

1.	Neurohypophysis (pars nervosa)
2.	Hormone-containing axons of hypothalamic neurons
3.	Capillaries surrounded by reticular tissue
4.	Nuclei of pituicytes
5.	Adenohypophysis (Anterior lobe, pars distalis)

Die blauen "Fasern" sind trotz Blaufärbung keine Bindegewebsfasern, sondern marklose Axone. Von den Pituizyten sieht man nur die Zellkerne, nicht aber die enge Beziehung ihres Zellleibes und ihrer Fortsätze zu den Axonen.

Legende:

	Neurohypophysis (pars nervosa)
	Hormone-containing axons of hypothalamic neurons
	Capillaries surrounded by reticular tissue
	Nuclei of pituicytes
	Adenohypophysis (Anterior lobe, pars distalis)

Endocrine System

Azan (Heidenhain)

Azan dye is a multiple or trichromatic staining method. It differentiates extracellular connective-tissue fibres, tissues, and cell components.

Structures	Colour
Nuclei	red
Cytoplasm	reddish
Connective-tissue fibres - Collagenic - Reticular - Elastic	blue blue unstained or reddish
Muscle tissue	red-orange

Dyes:

Azokarmin	Azokarmin is a red dye used mainly to stain cell nuclei.
Aniline blue- orange G	This dye is used for counterstaining.

Die Axone enthalten die Hormone Vasopressin oder Oxytoxin, die durch axoplasmatischen Transport in die Neurohypophyse gelangen. Wenn die neuroendokrinen Zellen durch ihren synaptischen Input erregt werden, werden die Hormone am Axonende freigesetzt und gelangen in die Kapillaren. Die Pituizyten sind eine Form von Astrozyten.

Magnification:

10x

Magnification:

26x

Magnification:

80x

Neurohypophysis (pars nervosa)

Hormone-containing axons of hypothalamic neurons

Capillaries surrounded by reticular tissue

Nuclei of pituicytes

Adenohypophysis (Anterior lobe, pars distalis)

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!