Specimen Nr. 04A

Specimen:

Pituitary gland (Rat)

Staining:

Anti prolactin

Magnification:

16x

Important structures :

1.Adenohypophysis with groups of prolcatin-secreting cells
2.Pars intermedia
3.Neurohypophysis
Die mammotropen Prolaktin-Zellen sind ebenso wie die anderen endokrinen Zellen weitgehend diffus in der Adenohypophyse verstreut. Durch Gegenfärbung mit Hämatoxylin sind daneben auch die Zellkerne der übrigen endokrinen Zellen des Vorder- und Mittellappens sowie diejenigen der Pituizyten im Hinterlappen blau gefärbt.

Legende:

Adenohypophysis with groups of prolcatin-secreting cells
Pars intermedia
Neurohypophysis

Endocrine System

Immunohistochemestry

Immunohistochemistry is used to confirm the presence of or to identify certain structures or substances in tissue sections which cannot be identified with conventional staining methods. Such structures include: cells, enzymes, hormones, macromolecules like nucleic acids and polysaccharides. The basis of immunohistochemical staining techniques is the antigen-antibody reaction. This method makes it possible to differentiate, for example, various cells in a tissue section according to their different metabolic products or surfaces. Either the metabolic product or a certain surface component serves as the antigen. In the first step, the antigen reacts with a specific antibody. The resulting antigen-antibody complex is invisible. Therefore, in a further step a second antibody bound to an adjuvant is added and binds to the initial antibody (so-called sandwich procedure). The bound adjuvant makes the antigen-antibody complex visible under the microscope and identifies the sought structure. Adjuvants are:

Combination with further dyes or staining techniques:

Prolaktin gehört zu den nicht-glandotropen Hormonen aus der Adenohypophyse, d.h. die Zielzellen sind selbst keine endokrinen Zellen. Die Zielzellen sind die exokrinen Zellen in der Brustdrüse. Die mammotropen Zellen werden durch einen hypothalamischen Prolactin-Inhibiting-Faktor gesteuert (inhibiert).
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Magnification:

16x

Magnification:

256x

Magnifications
Adenohypophysis with groups of prolcatin-secreting cells
Pars intermedia
Neurohypophysis

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

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