Hemoglobin is quickly degraded into hemosiderin in tissue. Methods used to confirm the presence of hemosiderin therefore also identify iron.
Prussian blue: | The Prussian-blue reaction provides histochemical evidence of trivalent iron, which appears as light-blue granules. |
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Turnbull blue | Iron, which is present in the body mainly in the form of trivalent iron, is reduced to bivalent iron and then identified as light-blue granules with Turnbull blue dye. |