Specimen Nr. 02B

Specimen:

Ovary (Cat)

Staining:

Azan

Magnification:

40x

Important structures :

1.Site of the original follicular cavity (with shrunken oocyte)
2.Granulosa lutein cells
3.Internal and external follicular theca
4.Blood vessel in thecal layer

Legende:

Site of the original follicular cavity (with shrunken oocyte)
Granulosa lutein cells
Internal and external follicular theca
Blood vessel in thecal layer

Corpus luteum[bo]

1. Former follicular antrum
2. Granulosa lutein cells
3. Theca lutein cells
4. Capillaries

Azan (Heidenhain)

Azan dye is a multiple or trichromatic staining method. It differentiates extracellular connective-tissue fibres, tissues, and cell components.

Structures Colour
Nuclei red
Cytoplasm reddish
Connective-tissue fibres
- Collagenic
- Reticular
- Elastic

blue
blue
unstained or reddish
Muscle tissue red-orange

Dyes:

Azokarmin Azokarmin is a red dye used mainly to stain cell nuclei.
Aniline blue-
orange G
This dye is used for counterstaining.
Home
Tutor
Help
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Boxes

Magnification:

4x

Magnification:

40x

Magnification:

64x

Magnifications
Site of the original follicular cavity (with shrunken oocyte)
Granulosa lutein cells
Internal and external follicular theca
Internal and external follicular theca
Blood vessel in thecal layer
Blood vessel in thecal layer
Blood vessel in thecal layer

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

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