Azan dye is a multiple or trichromatic staining method. It differentiates extracellular connective-tissue fibres, tissues, and cell components.
Structures | Colour |
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Nuclei | red |
Cytoplasm | reddish |
Connective-tissue fibres - Collagenic - Reticular - Elastic |
blue blue unstained or reddish |
Muscle tissue | red-orange |
Azokarmin | Azokarmin is a red dye used mainly to stain cell nuclei. |
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Aniline blue- orange G | This dye is used for counterstaining. |