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Specimen Nr. 05A

Specimen:

Skin of scrotum (Human being)

Staining:

Orcein

Magnification:

41x

Important structures :

1.Elastic fibres
2.Epidermis
3.Dermis
4.Papillary dermis
5.Reticular dermis
6.Keratinocytes with melanin granules (basal cell layer)
7.Keratinocytes (epidermis)
In dieser Abbildung sind die elastischen Fasern der menschlichen Skrotalhaut färberisch hervorgehoben. Man erkennt dickere und dünnere, längere und kürzere, teils leicht gewellt verlaufende Bruchstücke von elastischen Fasern. Sie sind braunrot angefärbt und verlaufen in den beiden Schichten der Dermis (Corium). In dem an die Epidermis grenzenden Stratum papillare der Dermis sind sie dünner und weniger zahlreich als im darunterliegenden Stratum reticulare. Neben den typischen Keratinozyten der oberen Epidermisschichten kommen im Stratum basale zahlreiche Keratinozyten vor, die Melaningranula enthalten. Das Pigment Melanin wird in den ebenfalls im Stratum basale liegenden, bei dieser Vergrößerung jedoch nicht klar zu identifizierenden Melanozyten produziert.

Legende:

Elastic fibres
Epidermis
Dermis
Papillary dermis
Reticular dermis
Keratinocytes with melanin granules (basal cell layer)
Keratinocytes (epidermis)

Loose connective tissue[kr]

1. Fibroblast
2. Fibrocyte
3. Single collagen microfibrils
4. Bundle of collagen microfibrils
5. Elastic fibres

Elastic Fibre Staining

A special staining technique used to identify elastic fibres. The table below shows the dyes which selectively stain elastic fibres.

Dyes

Colours of Elastic Fibres
Orcein

red-brown
Resorcin-fuchsin

blackish-blue to violet
Aldehydfuchsin

red to violet

Combinations with further dyes and staining techniques:

hemalaun

dye for staining cell nuclei
result: Cell nuclei turn intense blue.

nuclear fast red

used as a counterstain with Weigerts elastica dye
result: Cell nuclei turn red.

Die menschliche Haut besteht aus 3 Hauptschichten:
  1. Epidermis [Oberhaut]:
    Epithelgewebe
  2. Dermis (Corium) [Lederhaut]:
    Bindegewebe mit kollagenen und elastischen Fasern, Fibroblasten, Blutgefäßen, Nerven und sensiblen Nervenendigungen.
  3. Subcutis [Unterhaut]:
    Fettgewebe in variabler Menge
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Magnification:

41x

Magnification:

176x

Magnifications
Elastic fibres
Epidermis
Dermis
Papillary dermis
Reticular dermis
Keratinocytes with melanin granules (basal cell layer)
Keratinocytes (epidermis)

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3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

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