Die stärkere Vergrößerung des subepithalen lockeren faserigen Bindegewebes der Lippe zeigt die in verschiedenen Richtungen verlaufenden locker gepackten kollagenen und elastischen Fasern, die jedoch bei dieser Färbung nicht zu differenzieren sind. Zahlreiche Fibrozyten und freie Bindegewebszellen, wie Lymphozyten und Plasmazellen sind zu sehen. Charakteristisch sind weiterhin viele Blutgefäße.

Legende:

	Loose fibrous connective tissue with fibrocytes
	Loose fibrous connective tissue with fibrocytes and cells of the monuclear phagocyte system
	Blood vessel (with erythrocytes)

Hematoxylin and Eosin (H&E)

H&E is the most common survey stain, which shows cell nuclei and cytoplasmatic components.

Structures	Colour
Nuclei	blue
Cytoplasm	pale red
Connective-tissue fibres - Collagen - Reticular - Elastic	red - pale rose to red
Muscle tissue	red
Erythrocytes	red-orange

Dyes:

Hematoxylin

(e.g. hemalaun, Weigerts or Heidenhains iron-hematoxylin)
Hematoxylin is a positively charged dye (at low pH values). It therefore colors negatively charged (basophilic) structures blue, like the DNA of nuclear chromatin, the RNA of rough endoplasmatic reticulum, and the acidic glycosaminoglycans of hyaline cartilage.
Hematoxylin used in the preparation of iron varnish is Weigerts or Heidenhains iron-hematoxylin and in the preparation of aluminum varnish is hemalaun.

Eosin

Eosin is a negatively charged dye used for counterstaining. It stains all remaining structures different shades of red (acidophilic).

Thiazinrot

Thiazinrot behaves similar as eosin.

Lockeres faseriges Bindegewebe enthält die typischen Bindegewebskomponenten: ortsansässige und freie Bindegewebszellen, kollagene, elastische und in geringem Maß retikuläre Fasern sowie die lichtmikroskopisch strukturlose Grundsubstanz.

Das lockere faserige Bindegewebe ist im ganzen Körper weit verbreitet. Es liegt zwischen Organen und Organteilen (Lappen-, Läppchengliederung), zwischen Muskelfaserbündeln, um Gefäße und Nerven. Für viele Organe wie z.B. Leber, Nieren, Hoden liefert es das Stroma, das das spezifische Gewebe des Organs, auch Parenchym genannt, umgibt.

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

Specimen Nr. 04B

Specimen:

Staining:

Magnification:

Important structures :

Legende:

Hematoxylin and Eosin (H&E)

Dyes:

HistoNet2000 - Help