Den stabkernigen neutrophilen Granulozyten erkennt man an der Ausbildung C-förmiger Kernformen ohne erkennbare Schnürfurchen. Das Chromatin dieser Kerne ist grobschollig. Das Zytoplasma ist schwach eosinophil angefärbt mit feinsten, zart-violetten, neutrophilen Granula. Ein Nucleolus fehlt.

Legende:

	Neutrophilic granulocyte (band form)
	Segmented neutrophilic granulocyte
	Erythrocytes

Pappenheim stain

Pappenheim stain is a combination of May-Grünwald and Giemsa stains. They are the stains generally used for blood smears.

Structures	Colour
Nuclei	reddish-violet
Neutrophilic granules	bluish-pink to brownish
Eosinophilic granules	brick-red to brown-orange
Basophilic granules	dark blue-violet
Lymphocytic cytoplasm	light blue
Monocytic cytoplasm	light blue
Erythrocytes	pink
Thrombocytes	blue with red-violet internal bodies

Dyes:

The May-Grünwald stain contains the dyes eosin and methylene blue.
The Giemsa stain contains the dyes eosin, methylene blue, methylene azure and methylene violet.

55-70% der weißen Blutkörperchen sind neutrophile Granulozyten.

Beachte: Alle weißen Blutkörperchen machen nur ca. 1% der geformten Bestandteile des Blutes aus. Die hier angegebenen 55 ? 70% beziehen sich also auf dieses 1% der geformten Blutbestandteile!
Der Durchmesser der neutrophilen Granulozyten beträgt etwa 9-12µm und ist damit größer als bei Erythrozyten und Lymphozyten. Neutrophile Granulozyten enthalten drei verschiedene Arten von Granula, die weder eosinophil noch basophil sind.
Der sogenannte stabkernige Granulozyt ist der noch unsegmentierte direkte Vorläufer des segmentkernigen neutrophilen Granulozyten.

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

Specimen Nr. 04B

Specimen:

Staining:

Magnification:

Important structures :

Legende:

Pappenheim stain

Dyes:

HistoNet2000 - Help