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Specimen Nr. 01B

Specimen:

Skeletal striated muscle, Tongue (Cat)

Staining:

Hopa

Magnification:

28x

Important structures :

1.Skeletal striated muscle (cross section)
2.Skeletal striated muscle (sliced longitudinally)
3.Adipose cells
4.Arteriole
5.Interstitial connective tissue
6.Peripheral nerve fibres
Die quergestreiften Muskelfaserbündel sind quer und schräg angeschnitten. Das interstitielle Bindegewebe ist blau gefärbt und liegt zwischen den einzelnen Muskelbündeln. Es enthält zahlreiche Gefäße und Nerven.

Legende:

Skeletal striated muscle (cross section)
Skeletal striated muscle (sliced longitudinally)
Adipose cells
Arteriole
Interstitial connective tissue
Peripheral nerve fibres

Drawing of the transverse striations of myofibrils[bu]

AA band (anisotropic, light band)
II band (isotropic, dark band)
HH band
MM line
ZZ line


1. Sarcomere
2. Thin filament (mainly actin)
3. Thick filament (mainly myosin)

HOPA

Structures Colour
Nuclei brown
Cytoplasm grey-blue
Connective-tissue fibres
- Collagen
- Reticular
- Elastic
blue
blue
-
Myocytes grey-blue to grey-green
Erythrocytes yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Die Terminologie der Muskelzelle (Synonym: Muskelfaser) leitet sich vom griechischen Wort "Sarx" (Genitiv Sarcos) = "Fleisch" ab. Daher wird bei der Muskelzelle die Zellmembran als Sarkolemm, das Zytoplasma als Sarkoplasma und das endoplasmatische Retikulum als sarkoplasmatisches Retikulum bezeichnet. Auch der Begriff des Sarkomers, der funktionellen Einheit der kontraktilen Elemente (Myofibrillen) eines Muskels, leitet sich von diesem Wortstamm ab.
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Magnification:

11x

Magnification:

28x

Magnification:

70x

Magnification:

70x

Magnification:

400x

Magnifications
Skeletal striated muscle (cross section)
Skeletal striated muscle (sliced longitudinally)
Adipose cells
Arteriole
Interstitial connective tissue
Peripheral nerve fibres

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

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