Specimen Nr. 01C

Specimen:

Skeletal striated muscle, Tongue (Cat)

Staining:

Hopa

Magnification:

70x

Important structures :

1.Skeletal striated muscle fibres (sliced longitudinally)
2.Capillary
3.Arteriole
4.Interstitial connective tissue (endomysium)
5.Marginal muscle cell nuclei
6.Nuclei of fibrocytes
Der Längsschnitt der Zungenmuskulatur zeigt die parallel, dicht aneinanderliegenden Muskelfasern der quergestreiften Skelettmuskulatur. An der Zelloberfläche jeder Muskelfaser befinden sich hintereinanderliegend zahlreiche Zellkerne. Die durch die Myofibrillen bedingte Längsstreifung ist gut zu erkennen, ebenso in einigen Abschnitten die Querstreifung der Muskelfasern. Jede einzelne Muskelfaser wird von einem zarten, hier blau gefärbten, interstitiellen Bindegewebe, das auch als Endomysium bezeichnet wird, umgeben. Darin eingebettet, befinden sich Gefäße und Fibrozyten.

Legende:

Skeletal striated muscle fibres (sliced longitudinally)
Capillary
Arteriole
Interstitial connective tissue (endomysium)
Marginal muscle cell nuclei
Nuclei of fibrocytes

Drawing of the transverse striations of myofibrils[bu]

AA band (anisotropic, light band)
II band (isotropic, dark band)
HH band
MM line
ZZ line


1. Sarcomere
2. Thin filament (mainly actin)
3. Thick filament (mainly myosin)

HOPA

Structures Colour
Nuclei brown
Cytoplasm grey-blue
Connective-tissue fibres
- Collagen
- Reticular
- Elastic

blue
blue
-
Myocytes grey-blue to grey-green
Erythrocytes yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Jede Muskelfaser besteht aus hunderten einzelner kontraktiler Elemente, den Myofibrillen, die parallel zur Längsachse angeordnet sind. Die ineinander geschobenen dicken Myosin- und dünnen Actin- Filamente bilden die Grundbausteine einer Myofibrille, wobei 6 Actinfilamente ein Myosinfilament umgeben. Über Verdichtungszonen der Actinfilamente, Z-Streifen, sind die funktionellen Einheiten, die Sarkomer genannt werden, aneinander geknüpft. Innerhalb eines Sarkomers, das von einem Z-Streifen zum nächsten reicht, gibt es Bereiche (Streifen) in denen nur Actinfilamente (I-Streifen), nur Myosinfilamente, (H-Streifen) oder beide Proteinfilamente vorkommen (A-Streifen). Dieser unterschiedlichen Verteilung der Filamente liegt die Querstreifung sowohl der Herz- als auch der Skelettmuskulatur zugrunde.
Home
Tutor
Help
Exit
Boxes

Magnification:

11x

Magnification:

28x

Magnification:

70x

Magnification:

70x

Magnification:

400x

Magnifications
Skeletal striated muscle fibres (sliced longitudinally)
Skeletal striated muscle fibres (sliced longitudinally)
Skeletal striated muscle fibres (sliced longitudinally)
Capillary
Arteriole
Interstitial connective tissue (endomysium)
Marginal muscle cell nuclei
Marginal muscle cell nuclei
Nuclei of fibrocytes
Nuclei of fibrocytes

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

Cose help