| 1. | Villi |
| 2. | Crypts |
| 3. | Epithelial layer of mucosa (villus) |
| 4. | Lamina propria of mucosa of villus |
| 5. | Submucosa |
| 6. | Brunner (duodenal) glands |
 | Villi |
 | Crypts |
 | Epithelial layer of mucosa (villus) |
 | Lamina propria of mucosa of villus |
 | Submucosa |
 | Brunner (duodenal) glands |
|
Perjodic Acid Schiff Reaction:
Structures containing carbohydrates, like glycogen and reticular fibres containing lots of glycoprotein, basal membranes, and mucins stain pink to violet. Perjodic acid splits the carbon bonds between two neighboring secondary alcohols. Then the alcohol groups are oxidized to aldehydes and are stained reddish by Schiff reagent.
| Hemalaun / Toluidine blue / Aurantia | These dyes are used to stain cell nuclei. Result: Cell nuclei turn bright blue to dark blue, depending on the dye used. |
| Luxol fast blue | This dye is used to stain myelin sheaths. The dye has a special affinity to myelin. Result: Myelin sheaths turn bright blue, grey matter turns pale green, and cell nuclei turn dark blue |
 |
Magnification:
4x
Magnification:
10x
Magnification:
64x
1. Organization of the screen surface
Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)
2.Histologic specimen
Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.
3. Complementary information
Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated
4. General Program Functions
Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy
We hope you will enjoy working with HistoNet2000 and learn a lot from it!