Specimen Nr. 02C

Specimen:

Duodenum (Human being)

Staining:

PAS-hemalaun

Magnification:

64x

Important structures :

1.	Enterocytes with brush border
2.	Goblet cell
3.	Lamina propria of mucosa of villus
4.	Lymphatic vessel of lamina propria
5.	Lumen of small intestine

Der Ausschnitt zeigt mehrere nebeneinanderliegende Zotten. Die hohen Enterozyten des Schleimhautepithels zeigen zum Teil einen deutlichen Stäbchensaum. Zwischen diesen liegen die deutlich rot gefärbten Becherzellen. In der zellreichen Lamina propria unter dem Zottenepithel sind Ausschnitte von Lymphgefäßen zu sehen.

Legende:

	Enterocytes with brush border
	Goblet cell
	Lamina propria of mucosa of villus
	Lymphatic vessel of lamina propria
	Lumen of small intestine

Villi and crypts (longitudinal slice)[bo]

1.	Villi
2.	Intestinal crypts (glands of Lieberkühn)
3.	Simple columnar epithelium with microvilli (epithelial layer of mucosa)
4.	Goblet cells
5.	Lamina propria of mucosa
6.	Lamina muscularis of mucosa
7.	Lymphoid follicle
8.	Central lymhatic vessel
9.	Paneth's granular cells

PAS-Reaction

Perjodic Acid Schiff Reaction:

Structures containing carbohydrates, like glycogen and reticular fibres containing lots of glycoprotein, basal membranes, and mucins stain pink to violet. Perjodic acid splits the carbon bonds between two neighboring secondary alcohols. Then the alcohol groups are oxidized to aldehydes and are stained reddish by Schiff reagent.

Combinations with further dyes or staining techniques:

Hemalaun / Toluidine blue / Aurantia	These dyes are used to stain cell nuclei. Result: Cell nuclei turn bright blue to dark blue, depending on the dye used.
Luxol fast blue	This dye is used to stain myelin sheaths. The dye has a special affinity to myelin. Result: Myelin sheaths turn bright blue, grey matter turns pale green, and cell nuclei turn dark blue

Beschreibung mit Link und weiterem Text.

Magnification:

10x

Magnification:

64x

Enterocytes with brush border

Lamina propria of mucosa of villus

Lymphatic vessel of lamina propria

Lumen of small intestine

HistoNet2000 - Help

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Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
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