Specimen Nr. 01D

Specimen:

Mesenchyme (Rat fetus)

Staining:

Hopa

Magnification:

100x

Important structures :

1.	Mesenchymal cells
2.	Osseous progenitor cells
3.	Osteoblast with osteoid
4.	Osteoid
5.	Osteocyte in bone trabeculae
6.	Osteoclasts
7.	Bone matrix
8.	Primary bone marrow
9.	Blood vessel (lumen)
10.	Lymphatic vessel (lumen)

Der vergrößerte Ausschnitt der Unterkieferanlage läßt die verschiedenen Zelltypen erkennen, die bei der desmalen Ossifikation eine Rolle spielen:

Spindelförmige primitive Mesenchymzellen, die zu größeren Osteoprogenitorzellen (Knochenvorläuferzellen) werden,
Osteoblasten, die das hellblau gefärbte Osteoid bilden,
Osteozyten, die in kleinen Höhlen im Knochenbälkchen liegen,
Osteoklasten, kernreiche Riesenzellen.

Zwischen den Knochenbälkchen liegt zell- und gefäßreiches Mesenchym, das sog. primäre Knochenmark.

Legende:

	Mesenchymal cells
	Osseous progenitor cells
	Osteoblast with osteoid
	Osteoid
	Osteocyte in bone trabeculae
	Osteoclasts
	Bone matrix
	Primary bone marrow
	Blood vessel (lumen)
	Lymphatic vessel (lumen)

HOPA

Structures	Colour
Nuclei	brown
Cytoplasm	grey-blue
Connective-tissue fibres - Collagen - Reticular - Elastic	blue blue -
Myocytes	grey-blue to grey-green
Erythrocytes	yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Die Knochenbälkchen wachsen durch Apposition: an ihrer Oberfläche sind Osteoblasten aneinandergereiht, die stets einen mehr oder weniger breiten hellblau gefärbten Osteoidsaum erkennen lassen. Neben den Knochenaufbauvorgängen durch Osteoblasten spielen sich Knochenabbauvorgänge durch Osteoklasten ab.

Magnification:

16x

Magnification:

40x

Magnification:

100x

Magnification:

250x

Magnification:

100x

Osseous progenitor cells

Osteoblast with osteoid

Osteocyte in bone trabeculae

Blood vessel (lumen)

Lymphatic vessel (lumen)

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

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